Research

  Milton T. Stubbs

Prof. Stubbs’s research interests lie in the structural biology of proteins of therapeutic relevance, with a particular focus on macromolecular conformational transitions, including the influence of protein flexibility on ligand affinity.

Stubbs lab



  Jochen Balbach

Prof. Balbach’s research interests lie NMR spectroscopy, structural biology of proteins, function and dynamics of proteins, biophysical methods to study protein folding.

Balbach lab

  Daniel Huster   (now member of the scientific advisory board)

Prof. Huster studies the structure and dynamics of membrane proteins such as Ras or G protein-coupled receptors, the viscoelastic properties of natural and artificial biological tissues, and nano-biotechnologically modified lipid surfaces by solid-state NMR spectroscopy.

Huster lab at the University of Leipzig


Excerpt list of publications

Kolenko P, Koch B, Rahfeld JU, Schilling S, Demuth HU, Stubbs MT (2013) Crystal structure of the glutaminyl cyclase from Drosophila melanogaster in space group I4. Acta Cryst. F69, 358-361


Krug U, Patzschke R, Zebisch M, Balbach J, Sträter N (2013) Contribution of the two domains of E. coli 5’-nucleotidase to substrate specificity and catalysis. FEBS Lett 587, 460-466


Koch B, Kolenko P, Buchholz M, Carrillo DR, Parthier C, Wermann M, Rahfeld JU, Reuter G, Schilling S, Stubbs MT, Demuth HU (2012) Crystal structures of glutaminyl cyclases (QCs) from Drosophila melanogaster reveal active site conservation between insect and mammalian QCs. Biochemistry 51, 7383-7392


Drechsler N, Fröbel J, Jahreis G, Gopalswamy M, Balbach J, Bosse-Doenecke E, Rudolph R (2011) Binding specificity of the ectodomain of the parathyroid hormone receptor. Biophys Chem 154, 66-72


Klepsch MM, Kovermann M, Löw C, Balbach J, Permentier HP, Fusetti F, de Gier JW, Slotboom, DJ, Berntsson RP (2011) Escherichia coli peptide binding protein OppA has a preference for positively charged peptides. J Mol Biol 414, 75-85


Parthier C & Stubbs MT (2011) Peptide Hormone Recognition in Class B GPCRs: Role of the Extracellular Domain in Receptor Activation. In G Protein‐Coupled Receptors: From Structure to Function (J. Giraldo and J.‐P. Pin, eds.), Royal Society of Chemistry (Cambridge), pp. 75‐89


Ruiz‐Carrillo D, Koch B, Parthier C, Wermann M, Dambe T, Buchholz M, Ludwig HH, Heiser U, Rahfeld JU, Stubbs MT*, Schilling S, Demuth HU (2011) Structures of Glycosylated Mammalian Glutaminyl Cyclases Reveal Conformational Variability near the Active Center. Biochemistry 50, 6280‐6288


Theisgen S, Thomas L, Schröder T, Lange C, Kovermann M, Balbach J, Huster D (2011) The presence of membranes or micelles induces structural changes of the myristoylated guanylate-cyclase activating protein-2. Eur Biophys J 40, 565-576


Carrillo DR, Parthier C, Jänckel N, Grandke J, Stelter M, Schilling S, Boehme M, Neumann P, Wolf R, Demuth HU, Stubbs MT*, Rahfeld JU (2010) Kinetic and structural characterization of bacterial glutaminyl cyclases from Zymomonas mobilis and Myxococcus xanthus. Biol Chem 391, 1419‐1428


Dahse K, Garvey M, Kovermann M, Vogel A, Balbach J, Fändrich M, Fahr A (2010) DHPC strongly affects the structure and oligomerization propensity of Alzheimer’s Aβ(1-40) peptide. J Mol Biol 403, 643 659


Neumann P, Lieber D, Meyer S, Dautel P, Kerth A, Kraus I, Garten W, Stubbs MT (2009) Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals singlestranded RNA binding properties. PNAS USA 106, 3710‐3715


Parthier C, Runge S, Rudolph R, Stubbs MT (2009) Passing the baton in class B GPCRs: peptide hormone activation via helix induction? Trends Biochem Sci 34, 303‐310


Bosse-Doenecke E, Weininger U, Gopalswamy M, Balbach J, Knudsen SM, Rudolph R (2008) High yield production of recombinant native and modified peptides exemplified by ligands for G-protein coupled receptors. Protein Expr Purif 58, 114-121. Erratum in: Protein Expr Purif, 2010 70, 298


Löw C, Weininger U, Lee H, Schweimer K, Neundorf I, Beck-Sickinger AG, Pastor RW, Balbach J (2008) Structure and dynamics of helix‐0 of the N-BAR domain in lipid micelles and bilayers. Biophys J 95, 4315-4323


Neumann P, Weidner A, Pech A, Stubbs MT*, Tittmann K (2008) Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from Escherichia coli. PNAS 105, 17390‐17395 (*corresponding authors)


Weidner A, Neumann P, Wille G, Stubbs MT, Tittmann K (2008) Crystallization and preliminary X-ray diffraction analysis of full-length and proteolytically activated pyruvate oxidase from Escherichia coli. Acta Cryst F64, 179-181